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rabbit polyclonal anti syntaxin 18  (Proteintech)


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    Proteintech rabbit polyclonal anti syntaxin 18
    Rabbit Polyclonal Anti Syntaxin 18, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+syntaxin+18/Syntaxin+18+Antibody/pm40354525-310-38-44
    Average 93 stars, based on 8 article reviews
    rabbit polyclonal anti syntaxin 18 - by Bioz Stars, 2026-09
    93/100 stars

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    other:

    Article Title: Trehalose dimycolate inhibits phagosome maturation and promotes intracellular M. tuberculosis growth via noncanonical SNARE interactions.
    Article Snippet: Mouse monoclonal anti- Mincle (1:1,000) from MBL International Corporation, rabbit polyclonal antiserum for Endobrevin/VAMP8 (1:1,000 in 5% BSA in TBST; Synaptic Systems), rabbit polyclonal anti- Syntaxin 5 (1:1,000; Cell Signaling), rabbit monoclonal anti- Syntaxin 6 (1:1,000; Cell Signaling), rabbit polyclonal anti- Syntaxin 18 (1:1,000; Proteintech), rabbit monoclonal anti- GAPDH (1:1,000) from Cell Signaling.

    Article Title: Trehalose dimycolate inhibits phagosome maturation and promotes intracellular M. tuberculosis growth via noncanonical SNARE interaction
    Article Snippet: Mouse monoclonal anti-Mincle (1:1000) from MBL International Corporation, rabbit polyclonal antiserum for Endobrevin/VAMP8 (1:1000 in 5% Bovine Serum Albumin (BSA) in TBST; Synaptic Systems), rabbit polyclonal anti-Syntaxin 5 (1:1000; Cell Signaling), rabbit monoclonal anti-Syntaxin 6 (1:1000; Cell Signaling), rabbit polyclonal anti-Syntaxin 18 (1:1000; Proteintech), rabbit monoclonal anti-GAPDH (1:1000) from Cell Signaling.



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    rabbit polyclonal anti syntaxin 18  (Proteintech)
    93
    Proteintech rabbit polyclonal anti syntaxin 18
    Rabbit Polyclonal Anti Syntaxin 18, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+syntaxin+18/Syntaxin+18+Antibody/pm40354525-310-38-44
    Average 93 stars, based on 1 article reviews
    rabbit polyclonal anti syntaxin 18 - by Bioz Stars, 2026-09
    93/100 stars
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    rabbit polyclonal anti-syntaxin-18 -syntaxin-5  (Synaptic Systems)
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    Synaptic Systems rabbit polyclonal anti-syntaxin-18 -syntaxin-5
    Protease “degradomics” identifies SNAREs as SPP substrates. A, experimental outline of the SILAC-based MS analysis of high-salt, EDTA-washed, sodium carbonate–extracted membrane proteins. B, graphical representation of proteomics results showing -fold change in membrane proteins upon SPP WT expression from two replicates (mean heavy/light (H/L) ratio). Selected proteins are highlighted in red. C, cycloheximide (CHX) chase experiments showing turnover of ectopically expressed FLAG-tagged STX18 and <t>STX5</t> long form upon coexpression of SPP WT and catalytic mutant SPP-DA. Actin is used as a loading control. Quantifications are shown on the right. Error bars represent S.E. (n = 3). Arrows represent full-length (black) and cleaved (white) forms of the proteins. D, cycloheximide chase experiments in the presence of 2 μm epoxomicin or vehicle control (DMSO) shows that the N-terminal cleavage fragment of STX18 (white arrow) is degraded by the proteasome. Actin is used as a loading control. Quantifications are shown on the right. Error bars represent S.E. (n = 3). E, coimmunoprecipitation assays with ectopically expressed SPP-HA and endogenous STX18, STX5, and CLIMP63. The long form and short form of STX5 and monomer and SDS-stable dimer forms of SPP are indicated. The asterisk represents antibody heavy chain. IP, immunoprecipitation. F, treatment of HEK293T cells with 50 μm (Z-LL)2-ketone and the analysis of endogenous STX18 by Western blotting (wb). The bar graph shows quantifications of the steady-state level of STX18. Error bars represent S.E. (n = 6). Significant chance is indicated (**, p < 0.01, Student's t test). See Fig. S1E for data on biological replicates. G, cycloheximide chase of endogenous STX18 in the presence of 5 μm epoxomicin and the absence or presence of 50 μm (Z-LL)2-ketone, showing N-terminal cleavage fragment accumulation upon proteasome inhibition.
    Rabbit Polyclonal Anti Syntaxin 18 Syntaxin 5, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+syntaxin+18/rabbit+polyclonal+anti+syntaxin+1a+antiserum/pmc06393608-507-27-28
    Average 90 stars, based on 1 article reviews
    rabbit polyclonal anti-syntaxin-18 -syntaxin-5 - by Bioz Stars, 2026-09
    90/100 stars
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    rabbit polyclonal anti-syntaxin-18 and -syntaxin-5  (Synaptic Systems)
    90
    Synaptic Systems rabbit polyclonal anti-syntaxin-18 and -syntaxin-5
    Protease “degradomics” identifies SNAREs as SPP substrates. A, experimental outline of the SILAC-based MS analysis of high-salt, EDTA-washed, sodium carbonate–extracted membrane proteins. B, graphical representation of proteomics results showing -fold change in membrane proteins upon SPP WT expression from two replicates (mean heavy/light (H/L) ratio). Selected proteins are highlighted in red. C, cycloheximide (CHX) chase experiments showing turnover of ectopically expressed FLAG-tagged STX18 and <t>STX5</t> long form upon coexpression of SPP WT and catalytic mutant SPP-DA. Actin is used as a loading control. Quantifications are shown on the right. Error bars represent S.E. (n = 3). Arrows represent full-length (black) and cleaved (white) forms of the proteins. D, cycloheximide chase experiments in the presence of 2 μm epoxomicin or vehicle control (DMSO) shows that the N-terminal cleavage fragment of STX18 (white arrow) is degraded by the proteasome. Actin is used as a loading control. Quantifications are shown on the right. Error bars represent S.E. (n = 3). E, coimmunoprecipitation assays with ectopically expressed SPP-HA and endogenous STX18, STX5, and CLIMP63. The long form and short form of STX5 and monomer and SDS-stable dimer forms of SPP are indicated. The asterisk represents antibody heavy chain. IP, immunoprecipitation. F, treatment of HEK293T cells with 50 μm (Z-LL)2-ketone and the analysis of endogenous STX18 by Western blotting (wb). The bar graph shows quantifications of the steady-state level of STX18. Error bars represent S.E. (n = 6). Significant chance is indicated (**, p < 0.01, Student's t test). See Fig. S1E for data on biological replicates. G, cycloheximide chase of endogenous STX18 in the presence of 5 μm epoxomicin and the absence or presence of 50 μm (Z-LL)2-ketone, showing N-terminal cleavage fragment accumulation upon proteasome inhibition.
    Rabbit Polyclonal Anti Syntaxin 18 And Syntaxin 5, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+syntaxin+18/rabbit+polyclonal+anti+syntaxin+1a+antiserum/10__1074_slash_jbc__ra118__005642-279-26-27
    Average 90 stars, based on 1 article reviews
    rabbit polyclonal anti-syntaxin-18 and -syntaxin-5 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

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    Protease “degradomics” identifies SNAREs as SPP substrates. A, experimental outline of the SILAC-based MS analysis of high-salt, EDTA-washed, sodium carbonate–extracted membrane proteins. B, graphical representation of proteomics results showing -fold change in membrane proteins upon SPP WT expression from two replicates (mean heavy/light (H/L) ratio). Selected proteins are highlighted in red. C, cycloheximide (CHX) chase experiments showing turnover of ectopically expressed FLAG-tagged STX18 and STX5 long form upon coexpression of SPP WT and catalytic mutant SPP-DA. Actin is used as a loading control. Quantifications are shown on the right. Error bars represent S.E. (n = 3). Arrows represent full-length (black) and cleaved (white) forms of the proteins. D, cycloheximide chase experiments in the presence of 2 μm epoxomicin or vehicle control (DMSO) shows that the N-terminal cleavage fragment of STX18 (white arrow) is degraded by the proteasome. Actin is used as a loading control. Quantifications are shown on the right. Error bars represent S.E. (n = 3). E, coimmunoprecipitation assays with ectopically expressed SPP-HA and endogenous STX18, STX5, and CLIMP63. The long form and short form of STX5 and monomer and SDS-stable dimer forms of SPP are indicated. The asterisk represents antibody heavy chain. IP, immunoprecipitation. F, treatment of HEK293T cells with 50 μm (Z-LL)2-ketone and the analysis of endogenous STX18 by Western blotting (wb). The bar graph shows quantifications of the steady-state level of STX18. Error bars represent S.E. (n = 6). Significant chance is indicated (**, p < 0.01, Student's t test). See Fig. S1E for data on biological replicates. G, cycloheximide chase of endogenous STX18 in the presence of 5 μm epoxomicin and the absence or presence of 50 μm (Z-LL)2-ketone, showing N-terminal cleavage fragment accumulation upon proteasome inhibition.

    Journal: The Journal of Biological Chemistry

    Article Title: The intramembrane protease SPP impacts morphology of the endoplasmic reticulum by triggering degradation of morphogenic proteins

    doi: 10.1074/jbc.RA118.005642

    Figure Lengend Snippet: Protease “degradomics” identifies SNAREs as SPP substrates. A, experimental outline of the SILAC-based MS analysis of high-salt, EDTA-washed, sodium carbonate–extracted membrane proteins. B, graphical representation of proteomics results showing -fold change in membrane proteins upon SPP WT expression from two replicates (mean heavy/light (H/L) ratio). Selected proteins are highlighted in red. C, cycloheximide (CHX) chase experiments showing turnover of ectopically expressed FLAG-tagged STX18 and STX5 long form upon coexpression of SPP WT and catalytic mutant SPP-DA. Actin is used as a loading control. Quantifications are shown on the right. Error bars represent S.E. (n = 3). Arrows represent full-length (black) and cleaved (white) forms of the proteins. D, cycloheximide chase experiments in the presence of 2 μm epoxomicin or vehicle control (DMSO) shows that the N-terminal cleavage fragment of STX18 (white arrow) is degraded by the proteasome. Actin is used as a loading control. Quantifications are shown on the right. Error bars represent S.E. (n = 3). E, coimmunoprecipitation assays with ectopically expressed SPP-HA and endogenous STX18, STX5, and CLIMP63. The long form and short form of STX5 and monomer and SDS-stable dimer forms of SPP are indicated. The asterisk represents antibody heavy chain. IP, immunoprecipitation. F, treatment of HEK293T cells with 50 μm (Z-LL)2-ketone and the analysis of endogenous STX18 by Western blotting (wb). The bar graph shows quantifications of the steady-state level of STX18. Error bars represent S.E. (n = 6). Significant chance is indicated (**, p < 0.01, Student's t test). See Fig. S1E for data on biological replicates. G, cycloheximide chase of endogenous STX18 in the presence of 5 μm epoxomicin and the absence or presence of 50 μm (Z-LL)2-ketone, showing N-terminal cleavage fragment accumulation upon proteasome inhibition.

    Article Snippet: Antibodies The following antibodies were used: mouse monoclonal anti-FLAG (M2; Sigma-Aldrich), mouse monoclonal anti-HA (HA.11; Covance), mouse monoclonal anti-Myc (9B11; New England Biolabs), rabbit polyclonal anti-syntaxin-18 and -syntaxin-5 (Synaptic Systems), mouse monoclonal anti-CLIMP63 (G1/296; Enzo Life Sciences), mouse monoclonal anti-β actin (Sigma-Aldrich), and rabbit polyclonal anti-calnexin (Abcam).

    Techniques: Expressing, Mutagenesis, Immunoprecipitation, Western Blot, Inhibition